Our lab is interested in neuronal turnover, focusing on the mechanisms that regulate protein stability during brain development and aging
Previous and current research
Our overall goal is to elucidate the molecular principles underlying protein turnover in the brain with a specific focus on neuron development and aging. We are working on a range of labelling techniques including microscopy and mass spectrometry technologies to assay the stability and the replacement of proteins in vitro and in vivo in several model systems.
We have previously optimized pulse labelling in vivo and coupled it to mathematical modeling to study the turnover of brain proteins (Fornasiero et al. 2018). We are interested in the overall regulation mechanisms at the basis of protein turnover regulation, and we have found an interesting link between wobble nucleotide usage and protein stability (Mandad et al., 2018).
More in general we are interested in the molecular aspects regulating synaptic vesicle recycling, stability, trafficking and recycling during neuronal activity (Truckenbrodt et al., 2018).
Future projects and collaborations
We want to extend the use of metabolic labelling to other model organisms, biological conditions and explore the possibility of using pulse labelling for answering a more general set of dynamic issues that concern brain biology. Our close collaborators include Prof. Dr. Silvio O. Rizzoli (University Medicine Göttingen), Dr. Felipe Opazo (Center of Biostructural Imaging of Neurodegeneration, Göttingen) and Prof. Dr. Henning Urlaub (MPI for Biophysical Chemistry, Göttingen).